The former Bond girl, 62, and her 65-year-old partner have been separated for several months and are negotiating their divorce.
However, the former couple remains "devoted parents" to their children, 17-year-old twins John Stacy and Kristopher Steven.
A spokesperson for the pair states, "Jane Seymour and James Keach confirm that they are separated and have been for several months. At this time they are negotiating the terms of their divorce.
"They will continue their relationship as devoted parents to their children, as business associates and partners, and in their joint dedication to preserving and furthering the charitable endeavours that they've worked on throughout their marriage."
Seymour was married three times before Keach, while the actor was previously wed to two other women.